Marwa Emam, Akaber T. Keshta, Yasser M.A. Mohamed and Yasser A. Attia* Pages 169 - 186 ( 18 )
Background: Wound healing is a complex process necessary for repairing damaged tissues and preventing infection. Selenium nanoparticles (Se NPs) were known due to their antioxidant and antimicrobial effects, also niacin has angiogenesis and antioxidant effects that are important in wound healing.
Objective: The present study was conducted to investigate the effect of Se NPs and niacin in reducing and accelerating the wound healing time in mice.
Methods: A simple wet chemical method has been modified to synthesize Se NPs in order to investigate their effect and niacin on reducing the wound healing in 80 adult female albino mice (250 mm2 full thickness open excision wound) that were divided into eight groups (10 mice/each). After 30-days, the mice were sacrificed, blood and tissue samples were taken for analysis.
Results: The results showed that the percentage of wound area had been significantly reduced in Se NPs and niacin treated groups compared to the positive control. The level of Vascular Endothelial cell Growth Factor and Collagenase I in Se NPs and niacin groups significantly exceed those of other groups while Nitric Oxide (NO) was significantly decreased in treated groups. Liver and kidney functions showed the lower toxicity effect of Se NPs and niacin. Skin tissue showed the wound healing effect of Se NPs and niacin by regenerating skin layer compared to the positive group.
Discussion: The high antioxidant property and low hemolysis activity of Se NPs and niacin proved the biocompatible nature of Se NPs and niacin. A possible mechanism depending on the obtained results has been proposed.
Conclusion: Se NPs and niacin play an important role in accelerating and reducing the time of wound healing while they were antagonistic to each other.
Wound healing, selenium nanoparticles, ascorbic acid, niacin, antioxidant, biocombatability.
Chemistry Department, Faculty of Science, Zagazig University, Zagazig 44519, Chemistry Department, Faculty of Science, Zagazig University, Zagazig 44519, Photochemistry Department, National Research Center, Dokki, Giza 12622, National Institute of Laser Enhanced Sciences, Cairo University, Giza 12613