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Cytotoxicity Evaluation of Dimethoxy and Trimethoxy Indanonic Spiroisoxazolines Against Cancerous Liver Cells

Author(s):

Ahmad Abolhasani , Fatemeh Heidari , Somayeh Nouri, Shokoufeh Mousavi and Hoda Abolhasani *   Pages 1 - 10 ( 10 )

Abstract:


Background: 3'-(3,4-dimethoxyphenyl)-4'-(4-(methylsulfonyl)phenyl)-4'H-spiro[indene-2,5'-isoxazol]-1(3H)-one and 4'-(4-(methylsulfonyl)phenyl)-3'-(3,4,5-trimethoxyphenyl)-4'H-spiro[indene-2,5'-isoxazol]-1(3H)-one compounds containing indanonic spiroisoxazoline core are widely known for their antiproliferative activities and investigation of tubulin binding modes.

Objective: To evaluate the cytotoxicity effect of Dimethoxy and Trimethoxy Indanonic Spiroisoxazolines against HepG2 cancerous liver cell line and to perform a comparison with other known anti-liver cancer drugs.

Methods: The evaluation of cytotoxicity of dimethoxy and trimethoxy indanonic spiroisoxazoline compounds, Oxaliplatin, Doxorubicin, 5-fluorouracil and Cisplatin against HepG2 (hepatocellular liver carcinoma) cell line has been performed using MTT assay and analyzed by GraphPad PRISM software (version 8.0.2).

Results: Potent cytotoxicity effects against HepG2 cell line, comparable to Cisplatin (IC50= 0.047±0.0045 µM), Oxaliplatin (IC50= 0.0051µM), Doxorubicin (IC50= 0.0014µM) and 5-fluorouracil (IC50= 0.0089 µM), were shown by both dimethoxy (IC50= 0.059±0.012 µM) and trimethoxy (IC50= 0.086±0.019 µM) indanonic spiroisoxazoline compounds.

Conclusion: In vitro biological evaluations revealed that dimethoxy and trimethoxy indanonic spiroisoxazoline compounds are good candidates for development of new anti-liver cancer agents.

Keywords:

Anticancer, Indanonic Spiroisoxazoline, HepG2, Antitubulin, Selective COX-2 inhibitor, Cancerous Liver Cells.

Affiliation:

Spiritual Health Research Center, Qom University of Medical Sciences, Qom, Department of Anatomy, School of Medicine, Qom University of Medical Sciences, Qom, Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Spiritual Health Research Center, Qom University of Medical Sciences, Qom



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