Kristína Krajčíková, Gabriela Glinská and Vladimíra Tomečková* Pages 8 - 18 ( 11 )
Background: There are many different tools for diagnostics of various diseases. One of the simplest approach for the early, rapid and accurate diagnosis represents determination of biomarkers.
Focus: In the following text, we describe review of the latest discoveries in the field of biomarkers of selected diseases: intestinal ischemia and atherosclerosis. The aim of this review article is to show the problems which the researchers have been dealing with in the process of discovering and establishing novel biomarkers. This work describes the possibilities of monitoring biomarkers from noninvasive samples such as tears. Additionally, the actual possibilities of the spectroscopy techniques in monitoring and diagnostics of selected diseases are mentioned which might replace the need of biomarkers of several diseases.
Prospect: For the most diagnostic purposes, biomarkers should be analyzed in body fluid samples. For the biofluids, metabolic signatures could be determined, although there is no consensus on possible biomarkers yet. Metabolomics, the comprehensive, qualitative, and quantitative study of secondary metabolites and signaling molecules reveal a wide range of dysregulated molecules in various diseases. However, using spectroscopic methods could contribute to the traditional view on biomarkers by monitoring the relevant tissues and body fluid samples.
Biomarkers, spectroscopic methods, ischemia, atherosclerosis, tear fluid, glaucoma, dry eye, diabetic retinopathy.
Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Safarik University, Kosice, Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Safarik University, Kosice, Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Safarik University, Kosice